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Vol. 57. Issue 5.
Pages 338-344 (May 2021)
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Vol. 57. Issue 5.
Pages 338-344 (May 2021)
Original Article
Searching for Inflammatory and Oxidative Stress Markers Capable of Clustering Severe Asthma
Búsqueda de marcadores de estrés inflamatorio y oxidativo capaces de agrupar asma grave
Giovanna E. Carpagnanoa,1, Giulia Sciosciab,1,
Corresponding author

Corresponding author.
, Donato Lacedoniab, Piera Socciob, Carla M.I. Quaratob, Grazia Cotugnob, Maria G. Palumbob, Maria P. Foschino Barbarob
a Department of Basic Medical Sciences, Neuroscience and Sense Organs, University of Bari Aldo Moro, Bari, Italy
b Department of Medical and Surgical Sciences, Institute of Respiratory Diseases, University of Foggia, Policlinico “OO Riuniti”, Foggia, Italy
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Tables (4)
Table 1. Primers/probes sequences for MtDNA/nDNA determination using real time qPCR; mitochondrial DNA and β2M probes were labelled at 5′end with 6 FAM and MAX fluorescent dyes respectively, both probes contained BHQ-1 as a quencher dye at 3′end.
Table 2. Demographic, clinical and functional characteristics of study population.
Table 3. Inflammatory and oxidative-stress markers.
Table 4. Severe asthmatic endotypes.
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Asthma inflammation may feature an imbalance between oxidative stress and antioxidant defenses. Oxidative stress induces propagation of airways inflammation and corticosteroid insensitivity contributing to poor asthma control, and frequent severe acute exacerbations. This study assessed inflammation and oxidative stress in severe asthmatic subjects and evaluated the possible correlations between inflammatory and oxidative stress markers investigated and asthma severity.

Material and method

Fifty-three patients with severe asthma, 11 patients with mild-moderate asthma and 12 healthy subjects were enrolled and underwent fractional exhaled nitric oxide (FENO) analysis and blood and sputum count cell collection. The content of mitochondrial DNA (MtDNA) and nuclear DNA (nDNA) was measured in exhaled breath condensate (EBC) by Real Time PCR and the ratio between MtDNA/nDNA was calculated. We detected MtDNA/nDNA in the EBC of severe asthmatics.


We found higher exhaled MtDNA/nDNA in severe asthmatics respectively compared to mild-moderate ones and to healthy controls (10.4±2.2 vs 7.9±2.5, p<0.05 and 10.4±2.2 vs 6.51±0.21, p<0.05). The level of exhaled MtDNA/nDNA was significantly higher in Non-T2 endotype severe asthmatics than T2 (14.07±10. 8 vs 6.5±5.5, p<0.05).


Oxidative stress marker (MtDNA/nDNA) is increased significantly with asthma severity and may be useful for endotyping severe asthma.

Exhaled breath condensate
Mitochondrial DNA
Oxidative stress
Severe asthma

La inflamación en el asma puede presentar un desequilibrio entre el estrés oxidativo y las defensas antioxidantes. El estrés oxidativo induce la propagación de la inflamación de las vías aéreas y la insensibilidad a los corticosteroides, lo que contribuye a un control deficiente del asma y a frecuentes exacerbaciones agudas graves. Este estudio evaluó la inflamación y el estrés oxidativo en sujetos asmáticos graves y estudió las posibles correlaciones entre los marcadores de estrés inflamatorio y oxidativo investigados y la gravedad del asma.

Material y método

Se incluyó a 53 pacientes con asma grave, 11 pacientes con asma leve a moderada y 12 sujetos sanos, a los que se les realizó un análisis de fracción exhalada de óxido nítrico (FeNO) y un recuento celular del esputo y de sangre. Se midió el contenido de ADN mitocondrial (ADNmt) y ADN nuclear (ADNn) en el condensado de aire exhalado (CAE) mediante PCR en tiempo real y se calculó la ratio ADNmt/ADNn. Detectamos ADNmt/ADNn en el CAE de los asmáticos graves.


Encontramos unos niveles más altos de ADNmt/ADNn exhalados en los asmáticos graves en comparación con los leves moderados y los controles sanos (respectivamente, 10,4±2,2 frente a 7,9±2,5, p<0,05 y 10,4±2,2 frente a 6,51±0,21, p<0,05). El nivel de ADNmt/ADNn exhalado fue significativamente mayor en los asmáticos graves de endotipo no-T2 que en los T2 (14,07±10,8 frente a 6,5±5,5, p<0,05).


El marcador de estrés oxidativo (ADNmt/ADNn) aumenta significativamente con la gravedad del asma y puede ser útil para endotipar el asma grave.

Palabras clave:
Condensado de aire exhalado
ADN mitocondrial
Estrés oxidativo
Asma grave


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