Journal Information
Vol. 56. Issue 8.
Pages 532-534 (August 2020)
Vol. 56. Issue 8.
Pages 532-534 (August 2020)
Scientific Letter
Full text access
Bronchial Infection due to Pseudomonas Aeruginosa in Patients with Cystic Fibrosis Diagnosed in Neonatal Screening
Infección bronquial por Pseudomonas aeruginosa en los pacientes con fibrosis quística diagnosticados por cribado neonatal
Visits
943
Roser Ayats Vidal
Corresponding author
rayatsv@tauli.cat

Corresponding author.
, Montserrat Bosque García, Miguel García González, Óscar Asensio de la Cruz
Unidad de Fibrosis Quística y Unidad de Neumología, Alergia e Inmunología Pediátrica, Servicio de Medicina Pediátrica, Parc Taulí, Hospital Universitario de Sabadell, Sabadell (Barcelona), Spain
This item has received
Article information
Full Text
Bibliography
Download PDF
Statistics
Tables (2)
Table 1. Characteristics of Patients Diagnosed With Cystic Fibrosis by Newborn Screening and Those Not Diagnosed by Newborn Screening.
Table 2. Prevalence of Pseudomonas aeruginosa infection in patients diagnosed with cystic fibrosis by newborn screening and those not diagnosed by newborn screening.
Show moreShow less
Full Text
Dear Editor,

Chronic Pseudomonas aeruginosa lung infection is the most important risk factor for a poor respiratory outcome in patients with cystic fibrosis, and is associated with higher morbidity and mortality, which is worse the earlier it occurs.1–4

Eradication of the pathogen is essential to avoid chronic colonization, but this can only be achieved in the early stages, so it is essential that the infection is diagnosed early and treated intensively in order to prevent progression to chronicity.5–7

In Spain, newborn screening (NBS) began in 1999 in Catalonia, Castilla-León and the Balearic Islands, and in 2015 it was extended to all communities.8 The diagnosis of cystic fibrosis in the neonatal period allows the early detection of the primary bronchial infection with P. aeruginosa. If intensive and persistent treatment is established as soon as this pathogen is isolated, eradication can be achieved in most cases and chronic colonization can be delayed, improving survival.9–11

The main objective of this study was to describe the prevalence of primary infection, intermittent infection, and chronic infection with P. aeruginosa (according to Leeds criteria12) in patients with cystic fibrosis diagnosed by newborn screening (D-NBS) compared with patients not diagnosed by neonatal screening (ND-NBS) who are being or who have been followed up in our Pediatric Cystic Fibrosis Unit. The secondary objective was to describe the prevalence in both groups in terms of mutations.

This was a retrospective descriptive study that included all patients diagnosed with cystic fibrosis before the age of 18 years from 1985 to 2018. We compared D-NBS with ND-NBS patients (including false negatives on NBS). Patients were sub-classified as no function (NF): patients with 2 mutations with no or minimal CFTR function (group I, II or III mutations), and residual function (RF): patients with a mutation with no or minimal CFTR function and a mutation with residual CFTR function (group IV, V, VI, or VII mutation) or 2 mutations with residual CFTR function. Table 1 summarizes the characteristics of both patient groups.

Table 1.

Characteristics of Patients Diagnosed With Cystic Fibrosis by Newborn Screening and Those Not Diagnosed by Newborn Screening.

  D-NBS  ND-NBS 
Patients (n56  26 (2 false negatives per NBS) 
Mean age at diagnosis  1.5 months  4.38 (0.13–15) 
Current mean age in years  10.38 (0.8–18)  21.5 (5–36)a 
Sex n (%)  Male: 33 (58.9)Female: 23 (41.1)  Male: 13 (50)Female: 13 (50) 
Lung transplantation 
Deceased 
NF group n (%)  36 (64.28)  19 (73) 
RF group n (%)  20 (35.71)  7 (26.92) 

D-NBS: diagnosed by newborn screening; NBS: newborn screening; ND-NBS: not diagnosed by newborn screening; NF: no function, patients with 2 mutations with no or minimal CFTR function (group I, II or III mutations); RF: residual function, patients with a mutation with no or minimal CFTR function and a mutation with residual CFTR function (group IV, V, VI or VII mutation) or patients with 2 mutations with residual CFTR function.

a

Although the current age is 21.5 years, respiratory secretion cultures were only reviewed up to the age of 18.

Patients’ medical records and all cultures of respiratory secretions (from birth to age 18 or January 2018 if they were younger) were reviewed. Cultures were performed monthly and, when positive, weekly until negativization. The treatment used for P. aeruginosa primary bronchial infection was inhaled colistin, tobramycin, or aztreonam (3–6 months) along with oral ciprofloxacin (3 weeks). In chronic infections, continuous inhaled treatment with colistin or 28-day on-off cycles with tobramycin or aztreonam were prescribed. During mild-moderate exacerbations, treatment was oral ciprofloxacin (2–3 weeks) and in severe exacerbations, intravenous (β-lactam combined with an aminoglycoside according to sensitivity testing) therapy was administered.

From a statistical perspective, quantitative variables were described by mean and range, and qualitative variables by relative and absolute frequencies. The χ2 test was used to compare prevalences between groups.

Table 2 shows the prevalences of P. aeruginosa infection in both D-NBS and ND-NBS patients, and in the NF and RF patient subgroups.

Table 2.

Prevalence of Pseudomonas aeruginosa infection in patients diagnosed with cystic fibrosis by newborn screening and those not diagnosed by newborn screening.

  Definition According to Leeds12  D-NBS  ND-NBS  P 
Primary infection  First positive culture of P. aeruginosa  53.57% (30/56)By group:NF: 66.6% (24/36)RF: 30% (6/20)  92.30% (24/26)By group:NF: 89.4% (17/19)RF: 100% (7/7)  .0014 
Intermittent infection  Consecutive cultures intermittently positive and negative after initial infection. Microbiological criteria: ≤50% of cultures positive for P. aeruginosa in the previous 12 months  33.92% (19/56)By group:NF: 44.4% (16/36)RF: 15% (3/20)  76.92% (20/26)By group:NF: 84.2% (16/19)RF: 57.1% (4/7)  <.001 
Chronic infection  Cultures persistently positive for P. aeruginosa with no new clinical signs of infection and with inflammatory response. Microbiological criteria: >50% of cultures positive for P. aeruginosa in the previous 12 months  1.78% (1/56)By group:NF: 1.4% (1/36)RF: 0%  38.46% (10/26)By group:NF: 52.6% (10/19)RF: 0%  <.001 

They are also analyzed by patient subgroups: NF group: 2 mutations with no or minimal CFTR function (group I, II or III mutations), RF group: a mutation with no or minimal CFTR function and a mutation with residual CFTR function (group IV, V, VI, or VII mutation) or 2 mutations with residual CFTR function.

D-NBS: diagnosed by newborn screening; NBS: newborn screening; ND-NBS: not diagnosed by newborn screening; NF: no function; RF: residual function.

A comparison of the prevalences of P. aeruginosa infection between D-NBS and ND-NBS patients revealed statistically significant differences in primary infection (P=.0014), intermittent infection (P<.001), and chronic infection (P<.001). This may be due in part to age differences between the 2 groups, since the mean age of the D-NBS patients was 10.38 years and the ND-NBS patients were followed up to the age of 18 years.

Patients with more severe mutations (NF group) diagnosed by neonatal screening had a higher prevalence of primary P. aeruginosa infection than the RF group (P=.018). It should be noted that none of the patients in the RF group (neither D-NBS nor ND-NBS) had chronic P. aeruginosa infection.

Of the D-NBS patients, 53.57% (30/56) had P. aeruginosa infection at some time. Mean age at the time of primary infection was 6 years and the eradication rate was 100%. The median time between first and second infection was 29 months (range: 1.4–96.8). Only 1.78% (1/56) of this group had chronic infection.

Of the ND-NBS patients, 92.30% (24/26) had P. aeruginosa infection at some time. Overall, 19.23% (5/26) already had chronic infection at diagnosis, without eradication. The mean age at diagnosis of these 5 patients was 5.66 years, whereas the mean age at diagnosis for the whole group was 4.38 years. Another 19.23% (5/26) had intermittent infection at diagnosis, but this eventually became chronic. In total, 38.46% (10/26) of this group had chronic infection.

Our results are consistent with other publications, which also show a decrease in the prevalence of chronic P. aeruginosa infection after the introduction of neonatal screening.13–15 In Canada,13 the prevalence of chronic P. aeruginosa infection was 28.4% in D-NBS and 61.8% in ND-NBS patients (P<.001). In the United Kingdom,14 prevalence in children under 15 was 16% in the D-NBS and 20% in the ND-NBS group. The 2018 American Cystic Fibrosis Foundation registry shows that 46.2% of cystic fibrosis patients under the age of 18 had P. aeruginosa infection (17% intermittent and 28.3% chronic) with a median age at primary infection of 5.2 years.15 If we compare the results of these publications with ours, we can see that, although all of them show a decrease in the prevalence of chronic P. aeruginosa infection in D-NBS patients, prevalence in our series is lower. This may be due to very close follow-up, with monthly visits and respiratory secretion cultures, and early and intensive treatment of primary P. aeruginosa infection, and also because there are more patients in the RF group.

In conclusion, we observed a change in the natural history of P. aeruginosa bronchial infection in cystic fibrosis following the implementation of NBS, with a significant decrease (P<.001) in the prevalence of chronic infection with this pathogen in these patients.

Funding

The authors have not received any funding or grants.

Conflict of interests

The authors state that they have no conflict of interests.

References
[1]
S. Palser, S. Smith, E.F. Nash, A. Agarwal, A.R. Smyth.
Treatments for preventing recurrence of infection with Pseudomonas aeruginosa in people with cystic fibrosis.
Cochrane Database Syst Rev, 12 (2019), pp. CD012300
[2]
R. Cantón, A. Fernández, E. Gómez, R. Campo, M.A. Meseguer.
Infección bronquial crónica: el problema de Pseudomonas aeruginosa.
Arch Bronconeumol, 47 (2011), pp. 8-13
[3]
J. Milczewska, T. Wolkowicz, K. Zacharczuk, E. Mierzejewska, M. Kwiatkowska, K. Walicka-Serzysko, et al.
Clinical outcomes for cystic fibrosis patients with Pseudomonas aeruginosa cross-infections.
Pediatr Pulmonol, 55 (2020), pp. 161-168
[4]
M.D. Parkins, R. Somayaji, V.J. Waters.
Epidemiology biology, and impoact of clonal Pseudomonas aeruginosa infections in cystic fibrosis.
Clin Microbiol Rev, 31 (2018),
[5]
R. Cantón, L. Maíz, A. Escribano, C. Olveira, A. Olicer, O. Asensio, et al.
Consenso español para la prevención y el tratamiento de la infección bronquial por Pseudomonas aeruginosa en el paciente con fibrosis quística.
Arch Bronconeumol, 51 (2015), pp. 140-150
[6]
H. Lund-Palau, A.R. Turnbull, A. Bush, E. Bardin, L. Cameron, O. Soren.
Pseudomonas aeruginosa infection in cystic fibrosis: pathophysiological mechanisms and therapeutic approaches.
Expert Rev Respir Med, 10 (2016), pp. 685-697
[7]
S. Malhotra, D.J. Hayes, D.J. Wozniak.
Cystic fibrosis and Pseudomona aeruginosa: the host–microbe interface.
Clin Microbiol Rev, 32 (2019),
[8]
S. Gartner, N. Cobos.
Cribado neonatal para la fibrosis quística.
An Pediatr (Barc), 71 (2009), pp. 481-482
[9]
R. Cantón, N. Cobos, J. de Gracia, F. Baquero, J. Honorato, S. Gartner, en representación del Grupo Español de Consenso del Tratamiento Antimicrobiano en el Paciente con Fibrosis Quística, et al.
Consenso del tratamiento antimicrobiano en el paciente con fibrosis quística.
Arch Bronconeumol, 41 (2005), pp. 1-25
[10]
S. Gartner, A. Moreno, N. Cobos.
Tratamiento de la enfermedad respiratoria en la fibrosis quística.
Tratado de neumología pediátrica, pp. 849-866
[11]
P.A. Flume, B.P. O'Sullivan, K.A. Robinson, C.H. Goss, P.J. Mogayzel, D.B. Willey Courand, et al.
Cystic fibrosis pulmonary guidelines.
Am J Respir Crit Care Med, 176 (2007), pp. 957-969
[12]
M. Proesmans, W. Balinska-Miskiewicz, L. Dupont, X. Bossuyt, J. Verhaegen, N. Hoiby, et al.
Evaluating the ‘Leeds criteria’ for Pseudomonas aeruginosa infection in a cystic fibrosis centre.
Eur Respir J, 27 (2006), pp. 937-943
[13]
D.Y. Mak, J. Sykes, A.L. Stephenson, L.C. Lands.
The benefits of newborn screening for cystic fibrosis: the Canadian experience.
J Cyst Fibros, 15 (2016), pp. 302-308
[14]
D.K. Schlüter, K.W. Southern, C. Dryden, P. Diggle, D. Taylor-Robinson.
Impact of newborn screening on outcomes and social inequalities in cystic fibrosis: a UK CF registry-based study.
[15]
Annual Data Report 2018.
Cystic Fibrosis Foundation Patient Registry, (2018),

Please cite this article as: Ayats Vidal R, Bosque García M, García González M, Asensio de la Cruz Ó. Infección bronquial por Pseudomonas aeruginosa en los pacientes con fibrosis quística diagnosticados por cribado neonatal. Arch Bronconeumol. 2020;56:532–534.

Copyright © 2020. SEPAR
Archivos de Bronconeumología
Article options
Tools

Are you a health professional able to prescribe or dispense drugs?