TY - JOUR T1 - Core Microbiota in Central Lung Cancer With Streptococcal Enrichment as a Possible Diagnostic Marker JO - Archivos de Bronconeumología T2 - AU - Bello,Salvador AU - Vengoechea,José J. AU - Ponce-Alonso,Manuel AU - Figueredo,Ana L. AU - Mincholé,Elisa AU - Rezusta,Antonio AU - Gambó,Paula AU - Pastor,Juan Manuel AU - del Campo,Rosa SN - 03002896 M3 - 10.1016/j.arbres.2020.05.034 DO - 10.1016/j.arbres.2020.05.034 UR - https://www.archbronconeumol.org/en-core-microbiota-in-central-lung-articulo-S0300289620301927 AB - BackgroundDysbiosis in lung cancer has been underexplored. The aim of this study was to define the bacterial and fungal microbiota of the bronchi in central lung cancer and to compare it with that of the oral and intestinal compartments. MethodsTwenty-five patients with central lung cancer and sixteen controls without antimicrobial intake during the previous month were recruited. Bacterial and fungal distribution was determined by massive sequencing of bronchial biopsies and saliva and faecal samples. Complex computational analysis was performed to define the core lung microbiota. ResultsAffected and contralateral bronchi of patients have almost identical microbiota dominated by Streptococcus, whereas Pseudomonas was the dominant genera in controls. Oral and pulmonary ecosystems were significantly more similar in patients, probably due to microaspirations. Streptococcal abundance in the bronchi differentiated patients from controls according to a ROC curve analysis (90.9% sensitivity, 83.3% specificity, AUC=0.897). The saliva of patients characteristically showed a greater abundance of Streptococcus, Rothia, Gemella and Lactobacillus. The mycobiome of controls (Candida) was significantly different from that of patients (Malassezia). Cancer patients’ bronchial mycobiome was similar to their saliva, but different from their contralateral bronchi. ConclusionsThe central lung cancer microbiome shows high levels of Streptococcus, and differs significantly in its composition from that of control subjects. Changes are not restricted to tumour tissue, and seem to be the consequence of microaspirations from the oral cavity. These findings could be useful in the screening and even diagnosis of this disease. ER -